Analiza ilościowa wzorców metylacji DNA ma szczególne znaczenie w kilku sytuacjach rozwojowych i patologicznych. Konieczne jest opracowanie prostych i niezawodnych metod oceny metylacji DNA, aby ułatwić jej pomiar i interpretację w praktyce klinicznej. Opisujemy wysoce powtarzalną metodę CE-UV do rozdziału i wykrywania cytozyny i metylocytozyny po hydrolizie kwasem mrówkowym DNA wyekstrahowanego z ludzkiej krwi pełnej. Zhydrolizowane próbki suszono i kolejno rozpuszczano w wodzie, a następnie wstrzykiwano do kapilary bez procedur derywatyzacji próbek.
Zastosowanie buforu przebiegu zawierającego 50 mmol/l buforu fosforanowego BIS-TRIS propan (BTP) o pH 3,25 i 60 mmol/l buforu octanu sodu o pH 3,60 (4:1, v/v) pozwoliło na oddzielenie podstawowych analitów w ciągu 12 min. Testy precyzji wykazały podwyższoną powtarzalność przy CV między testami wynoszącym 1,98%.

Opracowanie metody błonowej do izolacji  genomowego  DNA  z  krwi ludzkiej  .

Wysoka jakość i wystarczająca ilość genomowego DNA (gDNA) są podstawowymi warunkami kilku zastosowań biologii molekularnej, w tym badań klinicznych związanych z genetyką, genomiką, polimorfizmem genów i odciskiem palca DNA. Krew pełna jest głównym źródłem gDNA w większości badań klinicznych. Obecnie do osiągnięcia tych celów wykorzystywane są przede wszystkim zestawy komercyjne. Jednak użycie zestawów jest ograniczone kosztem i zaangażowaniem kilku etapów wirowania. Inne opisane metody są albo pracochłonne, albo nie dają wysokiej jakości lub ilości gDNA lub obu. W tym miejscu przedstawiamy dane dotyczące opracowania niewymagającej wirowania, opłacalnej i przyjaznej dla użytkownika metody izolacji ludzkiego gDNA z kożuszka leukocytarnego ludzkiej krwi, która obejmuje ograniczoną liczbę kroków z około 15 minutami pracy rąk. na czas na próbkę.

Pig Blood Genomic DNA

PG-705 Zyagen 0.1mg 223 EUR

Blood Genomic DNA Kit

abx098868-100l Abbexa 100 µl 337.5 EUR

Blood Genomic DNA Kit

abx098868-1ml Abbexa 1 ml Ask for price

Blood Genomic DNA Kit

abx098868-200l Abbexa 200 µl Ask for price

Bovine Blood Genomic DNA

BG-705 Zyagen 0.1mg 223 EUR

Column-Pure Blood Genomic DNA Kit

D203-Mini-1 ABM 50 Preparations 105 EUR

Blood Genomic DNA Kit (0.1-20 ml)

abx098074-For200mlBlood Abbexa For 200 ml Blood 427.2 EUR

Blood Genomic DNA Kit (0.1-20 ml)

abx098074-For50mlBlood Abbexa For 50 ml Blood 260.4 EUR

Blood Genomic DNA Kit (5-250 ul)

20-abx098078 Abbexa
  • Ask for price
  • Ask for price
  • 200 rxns
  • 50 rxns

Blood Genomic DNA Kit (0.1-20 ml)

abx098074-100l Abbexa 100 µl 275 EUR

Blood Genomic DNA Kit (0.1-20 ml)

abx098074-1ml Abbexa 1 ml Ask for price

Blood Genomic DNA Kit (0.1-20 ml)

abx098074-200l Abbexa 200 µl 437.5 EUR

Blood Genomic DNA Kit (5-250 µl)

abx098078-100l Abbexa 100 µl 287.5 EUR

Blood Genomic DNA Kit (5-250 µl)

abx098078-1ml Abbexa 1 ml Ask for price

Blood Genomic DNA Kit (5-250 µl)

abx098078-200l Abbexa 200 µl 512.5 EUR

Rapid Blood Genomic DNA Extraction Kit

BT4782 Bio Basic 50Preps 91.97 EUR

Blood Genomic DNA Kit (No RNase A)

20-abx098864 Abbexa
  • Ask for price
  • Ask for price
  • 200 rxns
  • 50 rxns

Blood Genomic DNA Kit (No RNase A)

abx098864-100l Abbexa 100 µl 287.5 EUR

Blood Genomic DNA Kit (No RNase A)

abx098864-1ml Abbexa 1 ml Ask for price

Blood Genomic DNA Kit (No RNase A)

abx098864-200l Abbexa 200 µl 475 EUR

Blood Genomic DNA Kit (with Magnetic Stand)

abx098867-50rxns Abbexa 50 rxns 360 EUR

Blood Genomic DNA Kit (with Magnetic Stand)

abx098867-100l Abbexa 100 µl 375 EUR

Blood Genomic DNA Kit (with Magnetic Stand)

abx098867-1ml Abbexa 1 ml Ask for price

Blood Genomic DNA Kit (with Magnetic Stand)

abx098867-200l Abbexa 200 µl Ask for price

HiPurA® Blood Genomic DNA Miniprep Purif

MB504-20PR EWC Diagnostics 1 unit 34.21 EUR

HiPurA® Blood Genomic DNA Miniprep Purif

MB504-250PR EWC Diagnostics 1 unit 380.44 EUR

HiPurA® Blood Genomic DNA Miniprep Purif

MB504-50PR EWC Diagnostics 1 unit 81.65 EUR

HiPurA® Blood Genomic DNA Midiprep Purif

MB516-10PR EWC Diagnostics 1 unit 52.04 EUR

HiPurA® Blood Genomic DNA Midiprep Purif

MB516-25PR EWC Diagnostics 1 unit 123.24 EUR

HiPurA® Blood Genomic DNA Maxiprep Purif

MB517-10PR EWC Diagnostics 1 unit 93.49 EUR

HiPurA® Blood Genomic DNA Maxiprep Purif

MB517-25PR EWC Diagnostics 1 unit 230.27 EUR

HiPurA® 96 Blood Genomic DNA Purificatio

MB521-1X96PR EWC Diagnostics 1 unit 162.12 EUR

Insta NX® Blood Genomic DNA Purification

MBIN001-12PR EWC Diagnostics 1 unit 71.73 EUR

HiPer® Blood Genomic DNA Extraction Teac

HTBM006-10PR EWC Diagnostics 1 unit 38.51 EUR

HiPer® Blood Genomic DNA Extraction Teac

HTBM006-20PR EWC Diagnostics 1 unit 55.13 EUR

HiPer® Blood Genomic DNA Extraction Teac

HTBM007-10PR EWC Diagnostics 1 unit 36.65 EUR

HiPer® Blood Genomic DNA Extraction Teac

HTBM007-20PR EWC Diagnostics 1 unit 54.18 EUR

Blood Genomic DNA Extraction Mini Kit (100prep)

FABGK-100 Favorgen 100 preps 198 EUR

Blood Genomic DNA Extraction Mini Kit (300prep)

FABGK-300 Favorgen 300 preps 319.2 EUR

Blood Genomic DNA Extraction Mini Kit (100prep)

FABGK_100 Favorgen 1 kit 50.5 EUR

Blood Genomic DNA Extraction Mini Kit (300prep)

FABGK_300 Favorgen 1 kit 174 EUR

Magnetic Beads-Based Blood Genomic DNA Kit

abx460020-1096tests Abbexa 10 × 96 tests Ask for price

Magnetic Beads-Based Blood Genomic DNA Kit

abx460020-596tests Abbexa 5 × 96 tests Ask for price

Magnetic Beads-Based Blood Genomic DNA Kit

abx460020-96tests Abbexa 96 tests 437.5 EUR

Blood genomic DNA extraction and purification kit

K1443-100 Biovision each 574.8 EUR

96 Well Plate Blood Genomic DNA Miniprep Kit

BT92031 Bio Basic 2Plates, 192prep 210.85 EUR

96 Well Plate Blood Genomic DNA Miniprep Kit

BT92032 Bio Basic 5Plates, 480prep 389.9 EUR

Genomic DNA Mini Kit (Blood/Cultured Cell)

GB004 Geneaid Biotech each 3 EUR

Genomic DNA Mini Kit (Blood/Cultured Cell)

GB100 Geneaid Biotech each 70 EUR

Genomic DNA Mini Kit (Blood/Cultured Cell)

GB300 Geneaid Biotech each 189 EUR

Genomic DNA Maxi Kit (Blood/Cultured Cell)

GDM002 Geneaid Biotech each 21 EUR

Genomic DNA Maxi Kit (Blood/Cultured Cell)

GDM010 Geneaid Biotech each 89.3 EUR

Genomic DNA Maxi Kit (Blood/Cultured Cell)

GDM025 Geneaid Biotech each 199.5 EUR

Magnetic bead Blood/Cell Genomic DNA Kit

PDM06-0100 GeneOn 100 rcs 265 EUR

LumiPure genomic DNA Blood and Buccal Kit, 50

21553 Lumiprobe 50 preps 116.4 EUR

EZ-10 Spin Column Blood Genomic DNA Miniprep Kit

SK8253 Bio Basic 50Preps 100.08 EUR

EZ-10 Spin Column Blood Genomic DNA Miniprep Kit

SK8254 Bio Basic 100preps 144.86 EUR

Presto™ 96 Well Blood Genomic DNA Extraction Kit

96GBP02 Geneaid Biotech each 185 EUR

Presto™ 96 Well Blood Genomic DNA Extraction Kit

96GBP04 Geneaid Biotech each 340 EUR

Presto™ 96 Well Blood Genomic DNA Extraction Kit

96GBP10 Geneaid Biotech each 720 EUR

Magnetic Blood Genomic DNA Isolation Kit, 50 reactions/pk

208501 NEST Biotechnology 8 pcs/cs 74.91 EUR

Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus)

PDC09-0100 Bio-Helix 100 rxns 99 EUR

Blood Genomic DNA Extraction MIni Kit (Automatic Machine) Sample

FABGK-AUTO-024 Favorgen 24 preps 180 EUR

96 Well Human Adult Normal Genomic DNA Plate - Peripheral Blood Leukocyte

D8234148-1 Biochain 1 plate 292 EUR

Genomic DNA Isolation Reagent Kit(Blood/Cultured Cell/Tissue)

PDR05-0100 Bio-Helix 100 rxns 154 EUR

Magnetic Blood Spots Genomic DNA Isolation Kit, 50 reactions/pk

208401 NEST Biotechnology 8 pcs/cs 74.91 EUR

Genomic DNA - Human Adult Normal Tissue: Peripheral Blood Leukocyte, from a single donor

D1234148 Biochain 100 ug 329 EUR

Human Genomic DNA

BIO-35025 Bioline 500µl @ 200ng/µl Ask for price

Human Genomic DNA

PCR-261 Jena Bioscience GmbH 20µg 96 EUR

Human Genomic DNA 

X11000 EpiGentek
  • Ask for price
  • Ask for price
  • 0.2 ml
  • 0.2 ml

MINI GENOMIC DNA KIT-BLOOD/CC

IB47200 IBI Scientific 4 PREP SAMPLE KIT 30.11 EUR

MINI GENOMIC DNA KIT-BLOOD/CC

IB47201 IBI Scientific 100 PREP KIT 240.66 EUR

MINI GENOMIC DNA KIT-BLOOD/CC

IB47202 IBI Scientific 300 PREP KIT 653.6 EUR

MAXI GENOMIC DNA KIT-BLOOD/CC

IB47210 IBI Scientific 2/PREP SAMPLE KIT 53.67 EUR

MAXI GENOMIC DNA KIT-BLOOD/CC

IB47211 IBI Scientific 10/PREP KIT 292.21 EUR

MAXI GENOMIC DNA KIT-BLOOD/CC

IB47212 IBI Scientific 25/PREP KIT 630.66 EUR

Human Genomic DNA, male

GH-180M Zyagen 0.1mg 177 EUR

Human Skin Genomic DNA

HG-101 Zyagen 0.05mg 210 EUR

Human Lung Genomic DNA

HG-601 Zyagen 0.05mg 210 EUR

Human Brain Genomic DNA

HG-201 Zyagen 0.05mg 210 EUR

Human Colon Genomic DNA

HG-311 Zyagen 0.05mg 210 EUR

Human Liver Genomic DNA

HG-314 Zyagen 0.05mg 210 EUR

Human Heart Genomic DNA

HG-801 Zyagen 0.05mg 210 EUR

Human Brain Genomic DNA  

X11001 EpiGentek
  • Ask for price
  • Ask for price
  • 10 µg
  • 10 ul

Human Genomic DNA, female

GH-180F Zyagen 0.1mg 177 EUR

Human Testis Genomic DNA

HG-401 Zyagen 0.05mg 210 EUR

Human Uterus Genomic DNA

HG-411 Zyagen 0.05mg 210 EUR

Human Spleen Genomic DNA

HG-701 Zyagen 0.05mg 210 EUR

Human Thymus Genomic DNA

HG-702 Zyagen 0.05mg 210 EUR

Human Kidney Genomic DNA

HG-901 Zyagen 0.05mg 210 EUR

Human Stomach Genomic DNA

HG-302 Zyagen 0.05mg 210 EUR

Human Pancreas Genomic DNA

HG-313 Zyagen 0.05mg 210 EUR

Human Placenta Genomic DNA

HG-413 Zyagen 0.05mg 210 EUR

Human Esophagus Genomic DNA

HG-301 Zyagen 0.05mg 210 EUR

Human Intestine Genomic DNA

HG-306 Zyagen 0.05mg 210 EUR

Human Spinal Cord Genomic DNA

HG-230 Zyagen 0.05mg 210 EUR

Human Bone Marrow Genomic DNA

HG-704 Zyagen 0.05mg 319 EUR

Control Genomic DNA - Human Male

D1234999-G01 Biochain 100 ug 196 EUR

Blood Genomic DNA Extraction Mini Kit (50prep), (With Proteinase K Powder, for wide applications and simple procedure)

FABGK-001 Favorgen 50 preps 180 EUR

Blood Genomic DNA Extraction Mini Kit (100prep), (With Proteinase K Powder, for wide applications and simple procedure)

FABGK-001-1 Favorgen 100 preps 232.8 EUR

Blood Genomic DNA Extraction Mini Kit (300prep), (With Proteinase K Powder, for wide applications and simple procedure)

FABGK-001-2 Favorgen 300 preps 406.8 EUR

Blood Genomic DNA Extraction MAXI Kit (10prep) (With Proteinase K Powder, for wide applications and simple procedure)

FABGK-003 Favorgen 10 preps 242.4 EUR

Blood Genomic DNA Extraction MAXI Kit (24prep) (With Proteinase K Powder, for wide applications and simple procedure)

FABGK-003-1 Favorgen 24 preps 379.2 EUR

Blood Genomic DNA Extraction Mini Kit (50prep), (With Proteinase K Powder, for wide applications and simple procedure)

FABGK_001 Favorgen 1 kit 39.5 EUR

Blood Genomic DNA Extraction Mini Kit (100prep), (With Proteinase K Powder, for wide applications and simple procedure)

FABGK_001-1 Favorgen 1 kit 73 EUR

Blood Genomic DNA Extraction Mini Kit (300prep), (With Proteinase K Powder, for wide applications and simple procedure)

FABGK_001-2 Favorgen 1 kit 269 EUR

Blood Genomic DNA Extraction MAXI Kit (10prep) (With Proteinase K Powder, for wide applications and simple procedure)

FABGK_003 Favorgen 1 kit 80 EUR

Blood Genomic DNA Extraction MAXI Kit (24prep) (With Proteinase K Powder, for wide applications and simple procedure)

FABGK_003-1 Favorgen 1 kit 170 EUR

Control Genomic DNA - Human Female

D1234999-G02 Biochain 100 ug 196 EUR

Human Skeletal Muscles Genomic DNA

HG-102 Zyagen 0.05mg 210 EUR

Cat Genomic DNA

GC-130 Zyagen 0.1mg 177 EUR

Genomic DNA Kit

20-abx098076 Abbexa
  • Ask for price
  • Ask for price
  • 200 rxns
  • 50 rxns

ELK Genomic DNA

GE-240 Zyagen 0.1mg 177 EUR

Fig Genomic DNA

PLG-1042 Zyagen 0.1mg 307 EUR

Oat Genomic DNA

PLG-1096 Zyagen 0.1mg 307 EUR

Rye Genomic DNA

PLG-1097 Zyagen 0.1mg 307 EUR

Pea Genomic DNA

PLG-1141 Zyagen 0.1mg 307 EUR

GENOMIC DNA KIT

IB47250 IBI Scientific 2 X 96 PREP KIT 759.68 EUR

GENOMIC DNA KIT

IB47251 IBI Scientific 4 X 96 PREP KIT 1464.04 EUR

GENOMIC DNA KIT

IB47252 IBI Scientific 10 X 96 PREP KIT 3254.61 EUR

Genomic DNA Kit

abx098076-100l Abbexa 100 µl 300 EUR

Genomic DNA Kit

abx098076-1ml Abbexa 1 ml Ask for price

Genomic DNA Kit

abx098076-200l Abbexa 200 µl 550 EUR

AnaPrep Tissue DNA Extraction Kit (48) For extracting genomic DNA from a variety of animal tissues, swab, and blood stains

Z1322004 Biochain 1 kit (48 extractions) Including all required plastic disposables 259 EUR

Crab Genomic DNA

GRA-340 Zyagen 0.025mg 177 EUR

Goat Genomic DNA

GG-150 Zyagen 0.1mg 177 EUR

Clam Genomic DNA

GCL-325 Zyagen 0.025mg 177 EUR

Duck Genomic DNA

GD-220 Zyagen 0.1mg 177 EUR

Corn Genomic DNA

PLG-1002 Zyagen 0.1mg 307 EUR

Rice Genomic DNA

PLG-1004 Zyagen 0.1mg 307 EUR

Pear Genomic DNA

PLG-1033 Zyagen 0.1mg 307 EUR

Pork Genomic DNA

PCR-705 Jena Bioscience GmbH 20µg 90.1 EUR

Quail Genomic DNA

GQ-200 Zyagen 0.1mg 177 EUR

Squid Genomic DNA

GSQ-380 Zyagen 0.025mg 177 EUR

Camel Genomic DNA

GC-270 Zyagen 0.1mg 177 EUR

Goose Genomic DNA

GG-140 Zyagen 0.1mg 177 EUR

Ecoli Genomic DNA*

GE-310 Zyagen 0.05mg 177 EUR

Llama genomic DNA

GL-260 Zyagen 0.1mg 177 EUR

Yeast Genomic DNA*

GY-300 Zyagen 0.05mg 177 EUR

Apple Genomic DNA

PLG-1001 Zyagen 0.1mg 307 EUR

Beans Genomic DNA

PLG-1051 Zyagen 0.1mg 307 EUR

Lemon Genomic DNA

PLG-1062 Zyagen 0.1mg 307 EUR

Wheat Genomic DNA

PLG-1084 Zyagen 0.1mg 307 EUR

Onion Genomic DNA

PLG-1092 Zyagen 0.1mg 307 EUR

Maple Genomic DNA

PLG-1094 Zyagen 0.1mg 307 EUR

Lotus Genomic DNA

PLG-1161 Zyagen 0.1mg 307 EUR

Horse Genomic DNA

PCR-706 Jena Bioscience GmbH 20µg 90.1 EUR

Oyster Genomic DNA

GOY-330 Zyagen 0.025mg 177 EUR

Pigeon Genomic DNA

GP-210 Zyagen 0.1mg 177 EUR

Turkey Genomic DNA

GT-150 Zyagen 0.1mg 177 EUR

Alpaca Genomic DNA

GAP-260 Zyagen 0.1mg 177 EUR

Ferret Genomic DNA*

GF-180 Zyagen 0.05mg 177 EUR

Gerbil Genomic DNA*

GG-120 Zyagen 0.05mg 177 EUR

Shirmp Genomic DNA

GHR-375 Zyagen 0.025mg 177 EUR

Donkey Genomic DNA

GD-160 Zyagen 0.1mg 177 EUR

Orange Genomic DNA

PLG-1003 Zyagen 0.1mg 307 EUR

Cotton Genomic DNA

PLG-1022 Zyagen 0.1mg 307 EUR

Banana Genomic DNA

PLG-1031 Zyagen 0.1mg 307 EUR

Barley Genomic DNA

PLG-1041 Zyagen 0.1mg 307 EUR

Pepper Genomic DNA

PLG-1043 Zyagen 0.1mg 307 EUR

Potato Genomic DNA

PLG-1073 Zyagen 0.1mg 307 EUR

Tomato Genomic DNA

PLG-1074 Zyagen 0.1mg 307 EUR

Carrot Genomic DNA

PLG-1081 Zyagen 0.1mg 307 EUR

Radish Genomic DNA

PLG-1083 Zyagen 0.1mg 307 EUR

Cherry Genomic DNA

PLG-1091 Zyagen 0.1mg 307 EUR

Squash Genomic DNA

PLG-1111 Zyagen 0.1mg 307 EUR

Lentil Genomic DNA

PLG-1151 Zyagen 0.1mg 307 EUR

Ostrich Genomic DNA

GO-320 Zyagen 0.1mg 177 EUR

Catfish Genomic DNA

GFC-190 Zyagen 0.025mg 177 EUR

Lobster Genomic DNA

GLB-370 Zyagen 0.025mg 177 EUR

Mussels Genomic DNA

GMU-345 Zyagen 0.025mg 177 EUR

Apricot Genomic DNA

PLG-1011 Zyagen 0.1mg 307 EUR

Soybean Genomic DNA

PLG-1044 Zyagen 0.1mg 307 EUR

Spinach Genomic DNA

PLG-1054 Zyagen 0.1mg 307 EUR

Cabbage Genomic DNA

PLG-1071 Zyagen 0.1mg 307 EUR

Lettuce Genomic DNA

PLG-1072 Zyagen 0.1mg 307 EUR

Ginseng Genomic DNA

PLG-1088 Zyagen 0.05mg 307 EUR

Tobacco Genomic DNA

PLG-1101 Zyagen 0.1mg 307 EUR

Starfish Genomic DNA

GSF-390 Zyagen 0.025mg 177 EUR

Wildboar Genomic DNA

GW-250 Zyagen 0.1mg 177 EUR

Silkworm Genomic DNA*

GK-390 Zyagen 0.025mg 177 EUR

Crawfish Genomic DNA

GCF-405 Zyagen 0.025mg 177 EUR

Cucumber Genomic DNA

PLG-1032 Zyagen 0.1mg 307 EUR

Broccoli Genomic DNA

PLG-1061 Zyagen 0.1mg 307 EUR

Rapeseed Genomic DNA

PLG-1093 Zyagen 0.1mg 307 EUR

Flaxseed Genomic DNA

PLG-1095 Zyagen 0.1mg 307 EUR

Chickpea Genomic DNA

PLG-1121 Zyagen 0.1mg 307 EUR

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