Analiza ilościowa wzorców metylacji DNA ma szczególne znaczenie w kilku sytuacjach rozwojowych i patologicznych. Konieczne jest opracowanie prostych i niezawodnych metod oceny metylacji DNA, aby ułatwić jej pomiar i interpretację w praktyce klinicznej. Opisujemy wysoce powtarzalną metodę CE-UV do rozdziału i wykrywania cytozyny i metylocytozyny po hydrolizie kwasem mrówkowym DNA wyekstrahowanego z ludzkiej krwi pełnej. Zhydrolizowane próbki suszono i kolejno rozpuszczano w wodzie, a następnie wstrzykiwano do kapilary bez procedur derywatyzacji próbek.
Zastosowanie buforu przebiegu zawierającego 50 mmol/l buforu fosforanowego BIS-TRIS propan (BTP) o pH 3,25 i 60 mmol/l buforu octanu sodu o pH 3,60 (4:1, v/v) pozwoliło na oddzielenie podstawowych analitów w ciągu 12 min. Testy precyzji wykazały podwyższoną powtarzalność przy CV między testami wynoszącym 1,98%.

Opracowanie metody błonowej do izolacji  genomowego  DNA  z  krwi ludzkiej  .

Wysoka jakość i wystarczająca ilość genomowego DNA (gDNA) są podstawowymi warunkami kilku zastosowań biologii molekularnej, w tym badań klinicznych związanych z genetyką, genomiką, polimorfizmem genów i odciskiem palca DNA. Krew pełna jest głównym źródłem gDNA w większości badań klinicznych. Obecnie do osiągnięcia tych celów wykorzystywane są przede wszystkim zestawy komercyjne. Jednak użycie zestawów jest ograniczone kosztem i zaangażowaniem kilku etapów wirowania. Inne opisane metody są albo pracochłonne, albo nie dają wysokiej jakości lub ilości gDNA lub obu. W tym miejscu przedstawiamy dane dotyczące opracowania niewymagającej wirowania, opłacalnej i przyjaznej dla użytkownika metody izolacji ludzkiego gDNA z kożuszka leukocytarnego ludzkiej krwi, która obejmuje ograniczoną liczbę kroków z około 15 minutami pracy rąk. na czas na próbkę.

Blood Genomic DNA Extraction Mini Kit (100prep)

FABGK-100 Favorgen 100 preps 165 EUR

Blood Genomic DNA Extraction Mini Kit (300prep)

FABGK-300 Favorgen 300 preps 266 EUR

Blood Genomic DNA Kit (0.1-20 ml)

abx098074-For200mlBlood Abbexa For 200 ml Blood 356 EUR

Blood Genomic DNA Kit (0.1-20 ml)

abx098074-For50mlBlood Abbexa For 50 ml Blood 217 EUR

Blood Genomic DNA Kit (5-250 ul)

20-abx098078 Abbexa
  • 411.00 EUR
  • 230.00 EUR
  • 200 rxns
  • 50 rxns

Blood Genomic DNA Kit (No RNase A)

20-abx098864 Abbexa
  • 384.00 EUR
  • 230.00 EUR
  • 200 rxns
  • 50 rxns

Blood Genomic DNA Kit (with Magnetic Stand)

abx098867-50rxns Abbexa 50 rxns 300 EUR

Blood genomic DNA extraction and purification kit

K1443-100 Biovision 479 EUR

96 Well Plate Blood Genomic DNA Miniprep Kit

BT92031 Bio Basic 2Plates, 192prep 175.71 EUR

96 Well Plate Blood Genomic DNA Miniprep Kit

BT92032 Bio Basic 5Plates, 480prep 324.92 EUR

LumiPure genomic DNA Blood and Buccal Kit, 50

21553 Lumiprobe 50 preps 97 EUR

Human Genomic DNA

BIO-35025 Bioline 500µl @ 200ng/µl Ask for price

Human Genomic DNA

X11000 EpiGentek 0.2 ml Ask for price

EZ-10 Spin Column Blood Genomic DNA Miniprep Kit

SK8253 Bio Basic 50Preps 83.4 EUR

EZ-10 Spin Column Blood Genomic DNA Miniprep Kit

SK8254 Bio Basic 100preps 120.72 EUR

Blood Genomic DNA Extraction MIni Kit (Automatic Machine) Sample

FABGK-AUTO-024 Favorgen 24 preps 150 EUR

Human Brain Genomic DNA

X11001 EpiGentek 10 µg Ask for price

Genomic DNA - Human Male

D1234999-G01 Biochain 100 ug 188 EUR

Genomic DNA - Human Female

D1234999-G02 Biochain 100 ug 188 EUR

Genomic DNA Kit

20-abx098076 Abbexa
  • 439.00 EUR
  • 244.00 EUR
  • 200 rxns
  • 50 rxns

96 Well Human Adult Normal Genomic DNA Plate - Peripheral Blood Leukocyte

D8234148-1 Biochain 1 plate 256 EUR

Plant Genomic DNA Kit

20-abx098077 Abbexa
  • 411.00 EUR
  • 230.00 EUR
  • 200 rxns
  • 50 rxns

Bacteria Genomic DNA Kit

20-abx098080 Abbexa
  • 105.00 EUR
  • 244.00 EUR
  • 2 rxns
  • 50 rxns

Micro Genomic DNA Kit

20-abx098242 Abbexa
  • 105.00 EUR
  • 328.00 EUR
  • 2 rxns
  • 50 rxns

Plant Genomic DNA Kit

abx294004-50preps Abbexa 50 preps 398 EUR

Animal Genomic DNA Kit

abx294005-100preps Abbexa 100 preps 523 EUR

Animal Genomic DNA Kit

abx294005-50preps Abbexa 50 preps 398 EUR

Genomic DNA Isolation Kit

K2118-50 ApexBio 50 assays 321 EUR

Genomic DNA Isolation Kit

55R-1362 Fitzgerald 50 assays 385 EUR

Genomic DNA - Mouse Male

D1334999-G01 Biochain 100 ug 188 EUR

Genomic DNA - Mouse Female

D1334999-G02 Biochain 100 ug 188 EUR

Genomic DNA - Rat Male

D1434999-G01 Biochain 100 ug 188 EUR

Genomic DNA - Rat Female

D1434999-G02 Biochain 100 ug 188 EUR

Genomic DNA - Dog Male

D1734999-G01 Biochain 100 ug 188 EUR

Genomic DNA - Dog Female

D1734999-G02 Biochain 100 ug 188 EUR

Genomic DNA - Rabbit Male

D1834999-G01 Biochain 100 ug 188 EUR

Genomic DNA - Rabbit Female

D1834999-G02 Biochain 100 ug 188 EUR

Genomic DNA - Porcine Male

D1934999-G01 Biochain 100 ug 188 EUR

Genomic DNA - Porcine Female

D1934999-G02 Biochain 100 ug 188 EUR

Genomic DNA - Bovine Male

D1B34999-G01 Biochain 100 ug 188 EUR

Genomic DNA - Bovine Female

D1B34999-G02 Biochain 100 ug 188 EUR

Genomic DNA - Chicken Male

D1C34999-G01 Biochain 100 ug 188 EUR

Genomic DNA - Chicken Female

D1C34999-G02 Biochain 100 ug 188 EUR

Genomic DNA - Hamster Male

D1H34999-G01 Biochain 100 ug 188 EUR

Genomic DNA - Hamster Female

D1H34999-G02 Biochain 100 ug 188 EUR

Genomic DNA - Horse Male

D1O34999-G01 Biochain 100 ug 188 EUR

Genomic DNA - Horse Female

D1O34999-G02 Biochain 100 ug 188 EUR

Genomic DNA Extraction Kit

K5016005 Biochain 1 kit 329 EUR

Soil Genomic DNA Kit

K1411-250 Biovision 561 EUR

Soil Genomic DNA Kit

K1411-50 Biovision 316 EUR

Insect Genomic DNA Kit

K1412-250 Biovision 561 EUR

Insect Genomic DNA Kit

K1412-50 Biovision 316 EUR

Mollusc Genomic DNA Kit

K1413-250 Biovision 561 EUR

Mollusc Genomic DNA Kit

K1413-50 Biovision 316 EUR

Yeast Genomic DNA Kit

K1414-250 Biovision 561 EUR

Yeast Genomic DNA Kit

K1414-50 Biovision 316 EUR

Fungal Genomic DNA Kit

K1415-250 Biovision 561 EUR

Fungal Genomic DNA Kit

K1415-50 Biovision 316 EUR

Genomic DNA Isolation Kit

K281-50 Biovision 272 EUR

Fully CpG Methylated Human Genomic DNA

X10000 EpiGentek 10 µg/100 µl Ask for price

Blood Advance? DNA Polymerase

M1153-100 Biovision 224 EUR

Blood Advance? DNA Polymerase

M1153-400 Biovision 474 EUR

Genomic DNA from Human Adult Normal Tissue: Peripheral Blood Leukocyte, from a single donor

D1234148 Biochain 100 ug 282 EUR

Genomic DNA Extraction and Purification

EP10012 Neuromics 100 Tests 505 EUR

Genomic DNA Extraction KitAnimal Tissue

FYG102-100P Yeastern Biotech 100 Preps Ask for price

Genomic DNA Extraction KitAnimal Tissue

FYG102-300P Yeastern Biotech 300 Preps Ask for price

Genomic DNA Extraction KitAnimal Tissue

FYG102-50P Yeastern Biotech 50 Preps Ask for price

Genomic DNA Extraction Maxi KitPlant

FYG104-10P Yeastern Biotech 10 Preps Ask for price

Genomic DNA Extraction Maxi KitPlant

FYG104-25P Yeastern Biotech 25 Preps Ask for price

ISOLATE II Genomic DNA Kit

BIO-52065 Bioline 10 preps Ask for price

ISOLATE II Genomic DNA Kit

BIO-52066 Bioline 50 preps Ask for price

ISOLATE II Genomic DNA Kit

BIO-52067 Bioline 250 preps Ask for price

Plant Genomic DNA extraction buffer

20-abx098075 Abbexa
  • 286.00 EUR
  • 105.00 EUR
  • 100 ml
  • 1.2 ml

Marine Animal Genomic DNA Kit

20-abx098079 Abbexa
  • 105.00 EUR
  • 244.00 EUR
  • 2 rxns
  • 50 rxns

FFPE Tissue Genomic DNA Kit

abx098866-50rxns Abbexa 50 rxns 272 EUR

Zenoquick Genomic DNA Purification Kit

Z2003-200 GenDepot 200 preps 631 EUR

Zenoprep Genomic DNA Purification Kit

Z2004-400 GenDepot 400 preps 550 EUR

AccuRT Genomic DNA Removal Kit

G488 ABM 200 Reactions 82 EUR

Genomic DNA - Cynomolgus Monkey Male

D1534999-Cy-G01 Biochain 100 ug 188 EUR

Genomic DNA - Cynomolgus Monkey Female

D1534999-Cy-G02 Biochain 100 ug 188 EUR

Genomic DNA - Rhesus Monkey Male

D1534999-G01 Biochain 100 ug 188 EUR

Genomic DNA - Rhesus Monkey Female

D1534999-G02 Biochain 100 ug 188 EUR

Genomic DNA - Guinea Pig Male

D1G34999-G01 Biochain 100 ug 188 EUR

Genomic DNA - Guinea Pig Female

D1G34999-G02 Biochain 100 ug 188 EUR

Bacterial Genomic DNA Isolation Kit

K309-100 Biovision 376 EUR

100 BP DNA LADDER, 500UL PER KIT

M-DNA-100BP CORNING 1/pk 73 EUR

1 KB DNA LADDER, 500UL PER KIT

M-DNA-1KB CORNING 1/pk 70 EUR

FFPE Genomic DNA - Human Tumor Tissue: Breast

D2235086 Biochain 2 ug 821 EUR

FFPE Genomic DNA - Human Tumor Tissue: Colon

D2235090 Biochain 2 ug 821 EUR

FFPE Genomic DNA - Human Tumor Tissue: Lung

D2235152 Biochain 2 ug 821 EUR

FFPE Genomic DNA - Human Tumor Tissue: Pancreas

D2235188 Biochain 2 ug 821 EUR

FFPE Genomic DNA - Human Tumor Tissue: Stomach

D2235248 Biochain 2 ug 821 EUR

FFPE Genomic DNA - Human Tumor Tissue: Uterus

D2235274 Biochain 2 ug 821 EUR

ISOLATE II Blood DNA Kit

BIO-52062 Bioline 10 Preps Ask for price

ISOLATE II Blood DNA Kit

BIO-52063 Bioline 50 Preps Ask for price

AnaPrep Blood DNA Extraction Kit

Z1322001 Biochain 1 kit (48 extractions) Including all required plastic disposables 288 EUR

Whole Blood DNA Isolation Kit

K528-100 Biovision 376 EUR

Rapid Fungal Genomic DNA Isolation Kit

FT71415 Bio Basic 50Preps 76.64 EUR

Genomic DNA Extraction Mini KitPlant Tissue

FYG103-100P Yeastern Biotech 100 Preps Ask for price

Easy3-Plant Genomic DNA Isolation Kit

FYG503-100P Yeastern Biotech 100 Preps Ask for price

Easy3-Plant Genomic DNA Isolation Kit

FYG503-500P Yeastern Biotech 500 Preps Ask for price

Rapid Bacteria Genomic DNA Isolation Kit

BS8225 Bio Basic 50Preps 76.64 EUR

Rapid Yeast Genomic DNA Extraction Kit

BS8227 Bio Basic 50Preps 76.64 EUR

Rapid Animal Genomic DNA Extraction Kit

AT4780 Bio Basic 10Preps 69.03 EUR

Rapid Animal Genomic DNA Extraction Kit

AT4781 Bio Basic 50Preps 126.13 EUR

Rapid Animal Genomic DNA Extraction Kit

AT4782 Bio Basic 250preps 354.5 EUR

Genomic DNA Kit (No RNase A)

20-abx098862 Abbexa
  • 398.00 EUR
  • 230.00 EUR
  • 200 rxns
  • 50 rxns

Plant Tissue Genomic DNA Isolation Kit

K316-100 Biovision 376 EUR

Mammalian Cell Genomic DNA Isolation Kit

K967-100 Biovision 381 EUR

Genomic DNA from Human Tumor Cell Line: A431

D1255801 Biochain 100 ug 243 EUR

Genomic DNA from Human Tumor Cell Line: Hela

D1255811 Biochain 100 ug 243 EUR

Genomic DNA from Human Tumor Cell Line: Jurkat

D1255815 Biochain 100 ug 243 EUR

Genomic DNA from Human Tumor Cell Line: Raji

D1255840 Biochain 100 ug 243 EUR

FFPE Genomic DNA - Human Adult Normal Tissue: Colon

D2234090 Biochain 2 ug 599 EUR

FFPE Genomic DNA - Human Adult Normal Tissue: Liver

D2234149 Biochain 2 ug 599 EUR

FFPE Genomic DNA - Human Adult Normal Tissue: Lung

D2234152 Biochain 2 ug 599 EUR

FFPE Genomic DNA - Human Adult Normal Tissue: Pancreas

D2234188 Biochain 2 ug 599 EUR

VAHTS Human Genomic DNA Quantification and QC Kit

NQ201 Vazyme 300 rxn 575 EUR

AnaPrep Blood DNA Extraction Kit 1200

Z1322002 Biochain 1 kit (48 extractions) Including all required plastic disposables 490 EUR

Blood and Serum DNA Isolation Kit

K5017100 Biochain 1 kit 360 EUR

One-tube Plant Genomic DNA Extraction Kit

PT71816 Bio Basic 100preps 88.06 EUR

One-tube Plant Genomic DNA Extraction Kit

PT71817 Bio Basic 500Preps, 500prep 202.25 EUR

FavorPrep Genomic DNA Clean-Up Kit (50prep)

FAGDC-001 Favorgen 50 preps 129 EUR

FavorPrep Genomic DNA Clean-Up Kit (200prep)

FAGDC-001-1 Favorgen 200 preps 204 EUR

Plant Genomic DNA Extraction Mini Kit (50prep)

FAPGK-001 Favorgen 50 preps 158 EUR

Plant Genomic DNA Extraction Mini Kit (100prep)

FAPGK-001-1 Favorgen 100 preps 204 EUR

Plant Genomic DNA Extraction Mini Kit (200prep)

FAPGK-001-2 Favorgen 200 preps 274 EUR

Plant Genomic DNA Extraction Maxi Kit (10prep)

FAPGK-002 Favorgen 10 preps 201 EUR

Plant Genomic DNA Extraction Maxi Kit (24prep)

FAPGK-002-1 Favorgen 24 preps 317 EUR

Genomic DNA Extraction Mini KitBlood/Cultured Cells

FYG100-300P Yeastern Biotech 300 Preps Ask for price

Genomic DNA Extraction Mini KitBlood/Cultured Cells

FYG101-100P Yeastern Biotech 100 Preps Ask for price

gRazor, Genomic DNA Removal Enzyme Mix(5X)

R5800-100 GenDepot 100ul 300 EUR

gRazor, Genomic DNA Removal Enzyme Mix(5X)

R5800-500 GenDepot 500ul 1144 EUR

Mouse Tail Genomic DNA Extraction Kit(250)

M9100-250 GenDepot 250 prep 536 EUR

5-min. Genomic DNA Prep for PCR

P930-100 101Bio - Ask for price

5-min. Genomic DNA Prep for PCR

P930-5 101Bio - Ask for price

5-min. Genomic DNA Prep for PCR

P930-500 101Bio - Ask for price

One-Tube Bacteria Genomic DNA Isolation Kit

BS8413 Bio Basic 100preps 78.93 EUR

One-Tube Bacteria Genomic DNA Isolation Kit

BS8414 Bio Basic 500Preps, 500prep 165.71 EUR

One-4-All Genomic DNA Miniprep Kit

BS88503 Bio Basic 50Preps 116.99 EUR

One-4-All Genomic DNA Miniprep Kit

BS88504 Bio Basic 100preps 170.58 EUR

One-4-All Genomic DNA Miniprep Kit

BS88505 Bio Basic 250preps 327.96 EUR

Bacteria Genomic DNA Kit (No RNase A)

abx098240-50rxns Abbexa 50 rxns 230 EUR

Food and Fodder Security Genomic DNA Kit

abx098241-50rxns Abbexa 50 rxns 314 EUR

Plant Genomic DNA Kit(No RNase A)

20-abx098863 Abbexa
  • 384.00 EUR
  • 230.00 EUR
  • 200 rxns
  • 50 rxns

T-Pro Genomic DNA Midi Kit (20)

RB94-NGM020 T-Pro Biotechnology 20preps/kit 222 EUR

T-Pro Genomic DNA Mini Kit (100)

RB94-NGS100 T-Pro Biotechnology 100preps/kit 213 EUR

LumiPure genomic DNA from AnySample Kit, 50

21573 Lumiprobe 50 preps 108 EUR

Genomic DNA from Mouse Normal Tissue: Brain

D1334035 Biochain 100 ug 282 EUR

Genomic DNA from Mouse Normal Tissue: Heart

D1334122 Biochain 100 ug 282 EUR

Genomic DNA from Mouse Normal Tissue: Kidney

D1334142 Biochain 100 ug 282 EUR

Genomic DNA from Mouse Normal Tissue: Liver

D1334149 Biochain 100 ug 282 EUR

Genomic DNA from Mouse Normal Tissue: Lung

D1334152 Biochain 100 ug 282 EUR

Genomic DNA from Mouse Normal Tissue: Spleen

D1334246 Biochain 100 ug 282 EUR

Genomic DNA from Mouse Normal Tissue: Stomach

D1334248 Biochain 100 ug 282 EUR

Genomic DNA from Rat Normal Tissue: Brain

D1434035 Biochain 100 ug 282 EUR

Genomic DNA from Rat Normal Tissue: Heart

D1434122 Biochain 100 ug 282 EUR

Genomic DNA from Rat Normal Tissue: Kidney

D1434142 Biochain 100 ug 282 EUR

Genomic DNA from Rat Normal Tissue: Liver

D1434149 Biochain 100 ug 282 EUR

Genomic DNA from Rat Normal Tissue: Lung

D1434152 Biochain 100 ug 282 EUR

Genomic DNA from Rat Normal Tissue: Stomach

D1434248 Biochain 100 ug 282 EUR

Genomic DNA from Rat Normal Tissue: Testis

D1434260 Biochain 100 ug 282 EUR

Genomic DNA from Rat Normal Tissue: Thymus

D1434264 Biochain 100 ug 282 EUR

Genomic DNA from Plant Normal Tissue: Arabidopsis

D1634310 Biochain 10 ug 493 EUR

Genomic DNA from Plant Normal Tissue: Arabidopsis

D1634310-5 Biochain 5 ug 282 EUR

Genomic DNA from Plant Normal Tissue: Corn

D1634330 Biochain 10 ug 222 EUR

Genomic DNA from Plant Normal Tissue: Orange

D1634340 Biochain 10 ug 222 EUR

Genomic DNA from Plant Normal Tissue: Potato

D1634350 Biochain 10 ug 222 EUR

Genomic DNA from Plant Normal Tissue: Rice

D1634360 Biochain 10 ug 222 EUR

Genomic DNA from Plant Normal Tissue: Wheat

D1634390 Biochain 10 ug 222 EUR

96 Well Breast Tumor Genomic DNA Plate

D8235086-1 Biochain 1 plate 316 EUR

96 Well Colon Tumor Genomic DNA Plate

D8235090-1 Biochain 1 plate 316 EUR

96 Well Lung Tumor Genomic DNA Plate

D8235152-1 Biochain 1 plate 316 EUR

96 Well Stomach Tumor Genomic DNA Plate

D8235248-1 Biochain 1 plate 316 EUR

Genomic DNA from Human Tumor Cell Line: K 562

D1255820 Biochain 100 ug 243 EUR

Genomic DNA from Human Tumor Cell Line: MCF 7

D1255830 Biochain 100 ug 243 EUR

Blood Genomic DNA Extraction Mini Kit (50prep), (With Proteinase K Powder, for wide applications and simple procedure)

FABGK-001 Favorgen 50 preps 150 EUR

Blood Genomic DNA Extraction Mini Kit (100prep), (With Proteinase K Powder, for wide applications and simple procedure)

FABGK-001-1 Favorgen 100 preps 194 EUR

Blood Genomic DNA Extraction Mini Kit (300prep), (With Proteinase K Powder, for wide applications and simple procedure)

FABGK-001-2 Favorgen 300 preps 339 EUR

Blood Genomic DNA Extraction MAXI Kit (10prep) (With Proteinase K Powder, for wide applications and simple procedure)

FABGK-003 Favorgen 10 preps 202 EUR

Blood Genomic DNA Extraction MAXI Kit (24prep) (With Proteinase K Powder, for wide applications and simple procedure)

FABGK-003-1 Favorgen 24 preps 316 EUR

Blood DNA Extraction Kit (Proteinase K included)

BD05 GeneOn 5 preps Ask for price

Blood DNA Extraction Kit (Proteinase K included)

BD100 GeneOn 100 preps 141 EUR

Blood DNA Extraction Kit (Proteinase K included)

BD50 GeneOn 50 preps 100 EUR

Genorise® Blood DNA Extraction Kit 1000

GR101002 Genorise Scientific 1000 1150 EUR

Genorise® Blood DNA Extraction Kit 50

GR101019 Genorise Scientific 50 96 EUR

Genorise® Blood DNA Extraction Kit 100

GR101020 Genorise Scientific 100 159 EUR

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