Analiza ilościowa wzorców metylacji DNA ma szczególne znaczenie w kilku sytuacjach rozwojowych i patologicznych. Konieczne jest opracowanie prostych i niezawodnych metod oceny metylacji DNA, aby ułatwić jej pomiar i interpretację w praktyce klinicznej. Opisujemy wysoce powtarzalną metodę CE-UV do rozdziału i wykrywania cytozyny i metylocytozyny po hydrolizie kwasem mrówkowym DNA wyekstrahowanego z ludzkiej krwi pełnej. Zhydrolizowane próbki suszono i kolejno rozpuszczano w wodzie, a następnie wstrzykiwano do kapilary bez procedur derywatyzacji próbek.
Zastosowanie buforu przebiegu zawierającego 50 mmol/l buforu fosforanowego BIS-TRIS propan (BTP) o pH 3,25 i 60 mmol/l buforu octanu sodu o pH 3,60 (4:1, v/v) pozwoliło na oddzielenie podstawowych analitów w ciągu 12 min. Testy precyzji wykazały podwyższoną powtarzalność przy CV między testami wynoszącym 1,98%.

Opracowanie metody błonowej do izolacji  genomowego  DNA  z  krwi ludzkiej  .

Wysoka jakość i wystarczająca ilość genomowego DNA (gDNA) są podstawowymi warunkami kilku zastosowań biologii molekularnej, w tym badań klinicznych związanych z genetyką, genomiką, polimorfizmem genów i odciskiem palca DNA. Krew pełna jest głównym źródłem gDNA w większości badań klinicznych. Obecnie do osiągnięcia tych celów wykorzystywane są przede wszystkim zestawy komercyjne. Jednak użycie zestawów jest ograniczone kosztem i zaangażowaniem kilku etapów wirowania. Inne opisane metody są albo pracochłonne, albo nie dają wysokiej jakości lub ilości gDNA lub obu. W tym miejscu przedstawiamy dane dotyczące opracowania niewymagającej wirowania, opłacalnej i przyjaznej dla użytkownika metody izolacji ludzkiego gDNA z kożuszka leukocytarnego ludzkiej krwi, która obejmuje ograniczoną liczbę kroków z około 15 minutami pracy rąk. na czas na próbkę.

Blood Genomic DNA Kit (0.1-20 ml)

abx098074-For50mlBlood Abbexa For 50 ml Blood 260.4 EUR

Blood Genomic DNA Kit (5-250 ul)

20-abx098078 Abbexa
  • 493.20 EUR
  • 276.00 EUR
  • 200 rxns
  • 50 rxns

Rapid Blood Genomic DNA Extraction Kit

BT4782 Bio Basic 50Preps 91.97 EUR

Blood Genomic DNA Kit (No RNase A)

20-abx098864 Abbexa
  • 460.80 EUR
  • 276.00 EUR
  • 200 rxns
  • 50 rxns

Blood Genomic DNA Kit (with Magnetic Stand)

abx098867-50rxns Abbexa 50 rxns 360 EUR

Blood Genomic DNA Extraction Mini Kit (100prep)

FABGK-100 Favorgen 100 preps 198 EUR

Blood Genomic DNA Extraction Mini Kit (300prep)

FABGK-300 Favorgen 300 preps 319.2 EUR

96 Well Plate Blood Genomic DNA Miniprep Kit

BT92031 Bio Basic 2Plates, 192prep 210.85 EUR

96 Well Plate Blood Genomic DNA Miniprep Kit

BT92032 Bio Basic 5Plates, 480prep 389.9 EUR

Blood genomic DNA extraction and purification kit

K1443-100 Biovision each 574.8 EUR

LumiPure genomic DNA Blood and Buccal Kit, 50

21553 Lumiprobe 50 preps 116.4 EUR

EZ-10 Spin Column Blood Genomic DNA Miniprep Kit

SK8253 Bio Basic 50Preps 100.08 EUR

EZ-10 Spin Column Blood Genomic DNA Miniprep Kit

SK8254 Bio Basic 100preps 144.86 EUR

Blood Genomic DNA Extraction MIni Kit (Automatic Machine) Sample

FABGK-AUTO-024 Favorgen 24 preps 180 EUR

96 Well Human Adult Normal Genomic DNA Plate - Peripheral Blood Leukocyte

D8234148-1 Biochain 1 plate 254 EUR

Genomic DNA - Human Adult Normal Tissue: Peripheral Blood Leukocyte, from a single donor

D1234148 Biochain 100 ug 286 EUR

Human Genomic DNA

BIO-35025 Bioline 500µl @ 200ng/µl Ask for price

Human Genomic DNA 

X11000 EpiGentek
  • Ask for price
  • 235.40 EUR
  • 0.2 ml
  • 0.2 ml

Human Brain Genomic DNA  

X11001 EpiGentek
  • Ask for price
  • 77.00 EUR
  • 10 µg
  • 10 ul

Control Genomic DNA - Human Male

D1234999-G01 Biochain 100 ug 170 EUR

Genomic DNA Kit

20-abx098076 Abbexa
  • 526.80 EUR
  • 292.80 EUR
  • 200 rxns
  • 50 rxns

Blood Genomic DNA Extraction Mini Kit (50prep), (With Proteinase K Powder, for wide applications and simple procedure)

FABGK-001 Favorgen 50 preps 180 EUR

Blood Genomic DNA Extraction Mini Kit (100prep), (With Proteinase K Powder, for wide applications and simple procedure)

FABGK-001-1 Favorgen 100 preps 232.8 EUR

Blood Genomic DNA Extraction Mini Kit (300prep), (With Proteinase K Powder, for wide applications and simple procedure)

FABGK-001-2 Favorgen 300 preps 406.8 EUR

Blood Genomic DNA Extraction MAXI Kit (10prep) (With Proteinase K Powder, for wide applications and simple procedure)

FABGK-003 Favorgen 10 preps 242.4 EUR

Blood Genomic DNA Extraction MAXI Kit (24prep) (With Proteinase K Powder, for wide applications and simple procedure)

FABGK-003-1 Favorgen 24 preps 379.2 EUR

Control Genomic DNA - Human Female

D1234999-G02 Biochain 100 ug 170 EUR

AnaPrep Tissue DNA Extraction Kit (48) For extracting genomic DNA from a variety of animal tissues, swab, and blood stains

Z1322004 Biochain 1 kit (48 extractions) Including all required plastic disposables 216 EUR

AnaPrep Blood DNA Extraction Kit 1200 (48) For extracting genomic DNA from mammalian whole blood, peripheral mononuclear cell, or buffy coat. Sample volume range: up to 1 ml

Z1322002 Biochain 1 kit (48 extractions) Including all required plastic disposables 419 EUR

Soil Genomic DNA Kit

K1411-250 Biovision each 673.2 EUR

Soil Genomic DNA Kit

K1411-50 Biovision each 379.2 EUR

Plant Genomic DNA Kit

20-abx098077 Abbexa
  • 493.20 EUR
  • 276.00 EUR
  • 200 rxns
  • 50 rxns

Micro Genomic DNA Kit

20-abx098242 Abbexa
  • 126.00 EUR
  • 393.60 EUR
  • 2 rxns
  • 50 rxns

Plant Genomic DNA Kit

abx294004-50preps Abbexa 50 preps 477.6 EUR

Yeast Genomic DNA Kit

K1414-250 Biovision each 673.2 EUR

Yeast Genomic DNA Kit

K1414-50 Biovision each 379.2 EUR

Fully CpG Methylated Human Genomic DNA

X10000 EpiGentek 10 µg/100 µl Ask for price

AnaPrep Blood DNA Extraction Kit 200 (48) For extracting genomic DNA from mammalian whole blood, peripheral mononuclear cell, or buffy coat. Sample volume range: up to 400 µl

Z1322001 Biochain 1 kit (48 extractions) Including all required plastic disposables 227 EUR

Animal Genomic DNA Kit

abx294005-100preps Abbexa 100 preps 627.6 EUR

Animal Genomic DNA Kit

abx294005-50preps Abbexa 50 preps 477.6 EUR

Insect Genomic DNA Kit

K1412-250 Biovision each 673.2 EUR

Insect Genomic DNA Kit

K1412-50 Biovision each 379.2 EUR

Fungal Genomic DNA Kit

K1415-250 Biovision each 673.2 EUR

Fungal Genomic DNA Kit

K1415-50 Biovision each 379.2 EUR

Mollusc Genomic DNA Kit

K1413-250 Biovision each 673.2 EUR

Mollusc Genomic DNA Kit

K1413-50 Biovision each 379.2 EUR

Bacteria Genomic DNA Kit

20-abx098080 Abbexa
  • 126.00 EUR
  • 292.80 EUR
  • 2 rxns
  • 50 rxns

Genomic DNA Isolation Kit

55R-1362 Fitzgerald 50 assays 462 EUR

Genomic DNA Isolation Kit

K281-50 Biovision each 326.4 EUR

Genomic DNA Isolation Kit

K2118-50 ApexBio 50 assays 385.2 EUR

Genomic DNA - Human Tumor Cell Line: A431

D1255801 Biochain 100 ug 245 EUR

Genomic DNA - Human Tumor Cell Line: Hela

D1255811 Biochain 100 ug 245 EUR

Genomic DNA - Human Tumor Cell Line: Raji

D1255840 Biochain 100 ug 245 EUR

Genomic DNA - Human Tumor Cell Line: Jurkat

D1255815 Biochain 100 ug 245 EUR

FFPE Genomic DNA - Human Tumor Tissue: Lung

D2235152 Biochain 2 ug 960 EUR

FFPE Genomic DNA - Human Tumor Tissue: Colon

D2235090 Biochain 2 ug 960 EUR

FFPE Genomic DNA - Human Tumor Tissue: Breast

D2235086 Biochain 2 ug 960 EUR

FFPE Genomic DNA - Human Tumor Tissue: Uterus

D2235274 Biochain 2 ug 960 EUR

Genomic DNA - Human Tumor Cell Line: K 562

D1255820 Biochain 100 ug 245 EUR

FFPE Genomic DNA - Human Tumor Tissue: Stomach

D2235248 Biochain 2 ug 960 EUR

Genomic DNA - Human Tumor Cell Line: MCF 7

D1255830 Biochain 100 ug 245 EUR

FFPE Genomic DNA - Human Tumor Tissue: Pancreas

D2235188 Biochain 2 ug 960 EUR

ISOLATE II Genomic DNA Kit

BIO-52065 Bioline 10 preps Ask for price

ISOLATE II Genomic DNA Kit

BIO-52066 Bioline 50 preps Ask for price

ISOLATE II Genomic DNA Kit

BIO-52067 Bioline 250 preps Ask for price

FFPE Tissue Genomic DNA Kit

abx098866-50rxns Abbexa 50 rxns 326.4 EUR

Control Genomic DNA - Rat Male

D1434999-G01 Biochain 100 ug 170 EUR

Control Genomic DNA - Dog Male

D1734999-G01 Biochain 100 ug 170 EUR

Marine Animal Genomic DNA Kit

20-abx098079 Abbexa
  • 126.00 EUR
  • 292.80 EUR
  • 2 rxns
  • 50 rxns

Sera-Xtracta Genomic DNA Kit

29429140 Scientific Laboratory Supplies EACH 574.8 EUR

Control Genomic DNA - Mouse Male

D1334999-G01 Biochain 100 ug 170 EUR

Control Genomic DNA - Rat Female

D1434999-G02 Biochain 100 ug 170 EUR

Control Genomic DNA - Dog Female

D1734999-G02 Biochain 100 ug 170 EUR

Control Genomic DNA - Horse Male

D1O34999-G01 Biochain 100 ug 170 EUR

AccuRT Genomic DNA Removal Kit

G488 ABM 200 Reactions 98.4 EUR

Control Genomic DNA - Rabbit Male

D1834999-G01 Biochain 100 ug 170 EUR

Control Genomic DNA - Bovine Male

D1B34999-G01 Biochain 100 ug 170 EUR

Control Genomic DNA - Mouse Female

D1334999-G02 Biochain 100 ug 170 EUR

Control Genomic DNA - Porcine Male

D1934999-G01 Biochain 100 ug 170 EUR

Control Genomic DNA - Chicken Male

D1C34999-G01 Biochain 100 ug 170 EUR

Control Genomic DNA - Hamster Male

D1H34999-G01 Biochain 100 ug 170 EUR

Control Genomic DNA - Horse Female

D1O34999-G02 Biochain 100 ug 170 EUR

Genomic DNA Kit (No RNase A)

20-abx098862 Abbexa
  • 477.60 EUR
  • 276.00 EUR
  • 200 rxns
  • 50 rxns

FFPE Genomic DNA - Human Adult Normal Tissue: Lung

D2234152 Biochain 2 ug 684 EUR

Control Genomic DNA - Rabbit Female

D1834999-G02 Biochain 100 ug 170 EUR

Control Genomic DNA - Bovine Female

D1B34999-G02 Biochain 100 ug 170 EUR

FFPE Genomic DNA - Human Adult Normal Tissue: Colon

D2234090 Biochain 2 ug 684 EUR

FFPE Genomic DNA - Human Adult Normal Tissue: Liver

D2234149 Biochain 2 ug 684 EUR

VAHTS Human Genomic DNA Quantification and QC Kit

NQ201 Vazyme 300 rxn 690 EUR

Control Genomic DNA - Porcine Female

D1934999-G02 Biochain 100 ug 170 EUR

Control Genomic DNA - Chicken Female

D1C34999-G02 Biochain 100 ug 170 EUR

Control Genomic DNA - Hamster Female

D1H34999-G02 Biochain 100 ug 170 EUR

Plant Genomic DNA extraction buffer

20-abx098075 Abbexa
  • 343.20 EUR
  • 126.00 EUR
  • 100 ml
  • 1.2 ml

Bacterial Genomic DNA Isolation Kit

K309-100 Biovision each 451.2 EUR

Blood / Cultured Cells Genomic DNA Extraction Midi Kit (25prep) (With Proteinase K Powder, for wide applications and simple procedure)

FABGK-002 Favorgen 25 preps 241.2 EUR

Genomic DNA Extraction Maxi KitPlant

FYG104-10P Yeastern Biotech 10 Preps Ask for price

Genomic DNA Extraction Maxi KitPlant

FYG104-25P Yeastern Biotech 25 Preps Ask for price

FFPE Genomic DNA - Human Adult Normal Tissue: Pancreas

D2234188 Biochain 2 ug 684 EUR

Zenoprep Genomic DNA Purification Kit

Z2004-400 GenDepot 400 preps 660 EUR

Zenoquick Genomic DNA Purification Kit

Z2003-200 GenDepot 200 preps 757.2 EUR

Genomic DNA Extraction and Purification

EP10012 Neuromics 100 Tests 606 EUR

Genomic DNA Extraction KitAnimal Tissue

FYG102-100P Yeastern Biotech 100 Preps Ask for price

Genomic DNA Extraction KitAnimal Tissue

FYG102-300P Yeastern Biotech 300 Preps Ask for price

Genomic DNA Extraction KitAnimal Tissue

FYG102-50P Yeastern Biotech 50 Preps Ask for price

Genomic DNA - Rat Normal Tissue: Lung

D1434152 Biochain 100 ug 286 EUR

Genomic DNA - Rat Normal Tissue: Brain

D1434035 Biochain 100 ug 286 EUR

Genomic DNA - Rat Normal Tissue: Heart

D1434122 Biochain 100 ug 286 EUR

Genomic DNA - Rat Normal Tissue: Liver

D1434149 Biochain 100 ug 286 EUR

Control Genomic DNA - Guinea Pig Male

D1G34999-G01 Biochain 100 ug 170 EUR

T-Pro Genomic DNA Midi Kit (20)

RB94-NGM020 T-Pro Biotechnology 20preps/kit 266.4 EUR

T-Pro Genomic DNA Mini Kit (100)

RB94-NGS100 T-Pro Biotechnology 100preps/kit 255.6 EUR

Genomic DNA - Mouse Normal Tissue: Lung

D1334152 Biochain 100 ug 286 EUR

Genomic DNA - Rat Normal Tissue: Kidney

D1434142 Biochain 100 ug 286 EUR

Genomic DNA - Rat Normal Tissue: Testis

D1434260 Biochain 100 ug 286 EUR

Genomic DNA - Rat Normal Tissue: Thymus

D1434264 Biochain 100 ug 286 EUR

Genomic DNA - Plant Normal Tissue: Corn

D1634330 Biochain 10 ug 212 EUR

Genomic DNA - Plant Normal Tissue: Rice

D1634360 Biochain 10 ug 212 EUR

5-min. Genomic DNA Prep for PCR

P930-100 101Bio - Ask for price

5-min. Genomic DNA Prep for PCR

P930-5 101Bio - Ask for price

5-min. Genomic DNA Prep for PCR

P930-500 101Bio - Ask for price

Genomic DNA - Mouse Normal Tissue: Brain

D1334035 Biochain 100 ug 286 EUR

Genomic DNA - Mouse Normal Tissue: Heart

D1334122 Biochain 100 ug 286 EUR

Genomic DNA - Mouse Normal Tissue: Liver

D1334149 Biochain 100 ug 286 EUR

Genomic DNA - Rat Normal Tissue: Stomach

D1434248 Biochain 100 ug 286 EUR

Genomic DNA - Plant Normal Tissue: Wheat

D1634390 Biochain 10 ug 212 EUR

Control Genomic DNA - Guinea Pig Female

D1G34999-G02 Biochain 100 ug 170 EUR

Plant Genomic DNA Kit(No RNase A)

20-abx098863 Abbexa
  • 460.80 EUR
  • 276.00 EUR
  • 200 rxns
  • 50 rxns

Rapid Yeast Genomic DNA Extraction Kit

BS8227 Bio Basic 50Preps 91.97 EUR

Genomic DNA - Mouse Normal Tissue: Kidney

D1334142 Biochain 100 ug 286 EUR

Genomic DNA - Mouse Normal Tissue: Spleen

D1334246 Biochain 100 ug 286 EUR

Control Genomic DNA - Rhesus Monkey Male

D1534999-G01 Biochain 100 ug 170 EUR

Genomic DNA - Plant Normal Tissue: Orange

D1634340 Biochain 10 ug 212 EUR

Genomic DNA - Plant Normal Tissue: Potato

D1634350 Biochain 10 ug 212 EUR

Rapid Fungal Genomic DNA Isolation Kit

FT71415 Bio Basic 50Preps 91.97 EUR

Plant Tissue Genomic DNA Isolation Kit

K316-100 Biovision each 451.2 EUR

Rapid Animal Genomic DNA Extraction Kit

AT4780 Bio Basic 10Preps 82.84 EUR

Rapid Animal Genomic DNA Extraction Kit

AT4781 Bio Basic 50Preps 151.36 EUR

Rapid Animal Genomic DNA Extraction Kit

AT4782 Bio Basic 250preps 425.4 EUR

Genomic DNA - Mouse Normal Tissue: Stomach

D1334248 Biochain 100 ug 286 EUR

Easy3-Plant Genomic DNA Isolation Kit

FYG503-100P Yeastern Biotech 100 Preps Ask for price

Easy3-Plant Genomic DNA Isolation Kit

FYG503-500P Yeastern Biotech 500 Preps Ask for price

Rapid Bacteria Genomic DNA Isolation Kit

BS8225 Bio Basic 50Preps 91.97 EUR

Control Genomic DNA - Rhesus Monkey Female

D1534999-G02 Biochain 100 ug 170 EUR

Mammalian Cell Genomic DNA Isolation Kit

K967-100 Biovision each 457.2 EUR

Nucleon BACC3 Genomic DNA Extraction Kit

RPN8512 Scientific Laboratory Supplies EACH 484.8 EUR

Bacteria Genomic DNA Kit (No RNase A)

abx098240-50rxns Abbexa 50 rxns 276 EUR

96 Well Lung Tumor Genomic DNA Plate

D8235152-1 Biochain 1 plate 328 EUR

illustra blood genomicPrep Mini Spin Kit 50 prep

28904264 Scientific Laboratory Supplies EACH 134.4 EUR

illustra blood genomicPrep Mini Spin Kit 250 prep

28904265 Scientific Laboratory Supplies EACH 506.4 EUR

96 Well Colon Tumor Genomic DNA Plate

D8235090-1 Biochain 1 plate 328 EUR

Control Genomic DNA - Cynomolgus Monkey Male

D1534999-Cy-G01 Biochain 100 ug 170 EUR

Genomic DNA - Plant Normal Tissue: Arabidopsis

D1634310 Biochain 10 ug 547 EUR

Genomic DNA - Plant Normal Tissue: Arabidopsis

D1634310-5 Biochain 5 ug 286 EUR

Genomic DNA Extraction Mini KitPlant Tissue

FYG103-100P Yeastern Biotech 100 Preps Ask for price

One-4-All Genomic DNA Miniprep Kit

BS88503 Bio Basic 50Preps 140.39 EUR

One-4-All Genomic DNA Miniprep Kit

BS88504 Bio Basic 100preps 204.7 EUR

One-4-All Genomic DNA Miniprep Kit

BS88505 Bio Basic 250preps 393.55 EUR

96 Well Breast Tumor Genomic DNA Plate

D8235086-1 Biochain 1 plate 328 EUR

Control Genomic DNA - Cynomolgus Monkey Female

D1534999-Cy-G02 Biochain 100 ug 170 EUR

96 Well Stomach Tumor Genomic DNA Plate

D8235248-1 Biochain 1 plate 328 EUR

Food and Fodder Security Genomic DNA Kit

abx098241-50rxns Abbexa 50 rxns 376.8 EUR

Genomic DNA - Plant Normal Tissue: Soy bean

D1634370 Biochain 10 ug 212 EUR

Mouse Tail Genomic DNA Extraction Kit(250)

M9100-250 GenDepot 250 prep 643.2 EUR

gRazor, Genomic DNA Removal Enzyme Mix(5X)

R5800-100 GenDepot 100ul 360 EUR

gRazor, Genomic DNA Removal Enzyme Mix(5X)

R5800-500 GenDepot 500ul 1372.8 EUR

T-Pro Plant Genomic DNA Midi Kit (20)

RB94-PGM020 T-Pro Biotechnology 20preps/kit 266.4 EUR

T-Pro Plant Genomic DNA Mini Kit (100)

RB94-PGS100 T-Pro Biotechnology 100preps/kit 266.4 EUR

LumiPure genomic DNA from AnySample Kit, 50

21573 Lumiprobe 50 preps 129.6 EUR

FavorPrep Genomic DNA Clean-Up Kit (200prep)

FAGDC-001-1 Favorgen 200 preps 244.8 EUR

FavorPrep Genomic DNA Clean-Up Kit (50prep)

FAGDC-001 Favorgen 50 preps 154.8 EUR

One-tube Plant Genomic DNA Extraction Kit

PT71816 Bio Basic 100preps 105.67 EUR

One-tube Plant Genomic DNA Extraction Kit

PT71817 Bio Basic 500Preps, 500prep 242.7 EUR

Plant Genomic DNA Extraction Mini Kit (50prep)

FAPGK-001 Favorgen 50 preps 189.6 EUR

Plant Genomic DNA Extraction Mini Kit (100prep)

FAPGK-001-1 Favorgen 100 preps 244.8 EUR

Plant Genomic DNA Extraction Mini Kit (200prep)

FAPGK-001-2 Favorgen 200 preps 328.8 EUR

Plant Genomic DNA Extraction Maxi Kit (10prep)

FAPGK-002 Favorgen 10 preps 241.2 EUR

Plant Genomic DNA Extraction Maxi Kit (24prep)

FAPGK-002-1 Favorgen 24 preps 380.4 EUR

One-Tube Bacteria Genomic DNA Isolation Kit

BS8413 Bio Basic 100preps 94.72 EUR

One-Tube Bacteria Genomic DNA Isolation Kit

BS8414 Bio Basic 500Preps, 500prep 198.85 EUR

Genomic DNA - Rat Normal Tissue: Skeletal Muscle

D1434171 Biochain 100 ug 286 EUR

Genomic DNA - Rat Normal Tissue: Small Intestine

D1434226 Biochain 100 ug 286 EUR

Marine Animal Genomic DNA Kit (No RNase A)

abx298012-50rxns Abbexa 50 rxns 276 EUR

Genomic DNA - Human Tumor Tissue: Lung Tumor, from a single donor

D1235152 Biochain 50 ug 489 EUR

Genomic DNA - Mouse Normal Tissue: Skeletal Muscle

D1334171 Biochain 100 ug 286 EUR

Genomic DNA - Mouse Normal Tissue: Small Intestine

D1334226 Biochain 100 ug 286 EUR

Genomic DNA - Lupus: Lung, from a single donor

D1236152Lup Biochain 50 ug 504 EUR

Genomic DNA - Human Adult Normal Tissue: Lung, from a single donor

D1234152 Biochain 100 ug 286 EUR

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